Play as Eggplant Man:
Select story mode, then defeat Eggplant Man in level 1.

Play as Koopa Troopa:
Select story mode, then defeat Koopa Troopa in level 2.

Play as Gotcha-Wrench:
Select story mode, then defeat Gotcha-Wrench in level 3.

Play as Spike:
Select story mode, then defeat Spike in level 4.

Play as Koopa:
Select story mode, then defeat Koopa in level 5.

Play as Peach:
Successfully complete the game story mode. Peach will be unlocked after the ending sequence.

Play as Luigi:
Successfully complete the game story mode. Peach will be unlocked after the ending sequence.

Play as Rice Bread:
Select story mode, then defeat Rice Bread in level A.

Play as Ghost Girl:
Select story mode, then defeat Ghost Girl in level B.

Play as Carpenter:
Select story mode, then defeat Carpenter in level C.

Play as Ancient Robot:
Select story mode, then defeat Ancient Robot in level D.

Level A:
Successfully complete level 2 in story mode with a time consisting of the minutes equaling a multiple of your wins and seconds consisting of your opponents losses.

Level B:
Successfully complete level 3 in story mode with a time consisting of the minutes equaling a multiple of your wins and seconds consisting of your opponents losses.

Level C:
Successfully complete level 4 in story mode with a time consisting of the minutes equaling a multiple of your wins and seconds consisting of your opponents losses.

Level D:
Successfully complete level C in story mode.

Level 5 Rematch:
Successfully complete level D in story mode.

Hidden ending:
Successfully complete Level 5 Rematch in story mode.